Composition of Cell Wall Polysaccharides and Differences in Pectin Activity Between the Peel and Pulp of Goji berry
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Abstract:
To investigate the differences in composition and activity of pectin polysaccharides from the cell walls of Goji berry peel and flesh, the pectin polysaccharides from the both sources were extracted. The basic structural characteristics of the pectin polysaccharides from the cell walls of the peel and flesh were analyzed using UV-Vis spectrophotometry and Fourier-transform infrared spectroscopy; anion exchange chromatography and gel permeation chromatography were used to determine the monosaccharide composition and molecular weight distribution, and the inhibitory effects of pectic polysaccharides from peel and flesh cell wall on pancreatic lipase and α-amylase were measured by enzyme inhibition experiments. The results showed that the content of pectin polysaccharides in the Goji berry peel was significantly higher than that in the flesh (P < 0.05); both peel and flesh pectin polysaccharides were primarily composed of arabinose, galactose, galacturonic acid, rhamnose, xylose, and glucose, with the peel pectin exhibiting a higher molecular weight. The semi inhibitory concentration (IC50) of the flesh pectin against pancreatic lipase was 4.334 mg/mL, showing a better inhibitory effect than that of the peel; the peel pectin demonstrated a superior inhibitory effect on α-amylase (IC50 of 6.939 mg/mL) compared to the flesh cell wall pectin, with both exhibiting mixed-type inhibition, indicating that both peel and flesh pectin polysaccharides have the potential to regulate the body's glycolipid metabolism. The research findings provided theoretical basis and development ideas for the high-value utilization of by-products derived from the processing Goji berry.
