Long-chain Inulin Ameliorates Inflammation and Gut Microbiota Dysbiosis in Aging and Eczema Mice
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Abstract:
To investigate the potential mechanisms behind the improving effect of long-chain inulin against eczematous dermatitis in aging mice. The improving effects of 5 % and 20 % long-chain inulin on eczema in aging mice model induced by D-galactose combined with DNCB (2,4-Dinitrochlorobenzene) was analyzed after feeding for 3 weeks. Herein, pathological changes of skin and colon tissues, inflammation-related factors levels of serum, spleen, liver, thymus, and colon tissues, as well as fecal microbiota were evaluated. The results showed that low-dose and high-dose long-chain inulin intervention alleviated the skin damage and improved the pathological changes of colon. The epidermal thickness decreased by 32.07% and 22.63% in the low-dose and high-dose long-chain inulin groups, respectively. The mRNA expressions of IL-1β (Interleukin-1 Beta) and IL-6 (Interleukin-6) in colon were significantly decreased, as well as the levels of IL-6 and IL-10 (Interleukin-10) in serum, spleen, liver, and thymus were effectively reduced after low-dose and high-dose long-chain inulin intervention. The results of 16S rRNA sequencing showed that the relative abundance of Verrucomicrobia, Verrucomicrobiaceae, Akkermansia and Clostridiuma was significantly increaseed in the model group mice, while different doses of long-chain inulin intervention shifted these changes back to normal levels. In comparison with the model group, the relative abundance of Bacteroidetes, Bacteroidaceae, and Bacteroides significantly promoted, and the relative abundance of Firmicutes, Lactobacillaceae, and Lactobacillus significantly decreased after high-dose long-chain inulin intervention. The results will provide theoretical basis for the application of long-chain inulin dietary intervention in age-related eczematous dermatitis.
