Construction of beeswax-based Ganoderma lucidum polysaccharide emulsion gel system and optimisation of anti-fatigue function
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Abstract:
Common margarine increases cardiovascular and cerebrovascular prevalence due to high trans fats and excessive intake. To improve this situation, in this study, beeswax was chosen as the wall material to encapsulate Ganoderma lucidum polysaccharides, and a Ganoderma lucidum polysaccharides emulsion gel with anti-fatigue effect was developed as a cream improver. The emulsion gel properties were determined using a zeta potential meter and laser granulometry, among other methods. It was confirmed that the Ganoderma lucidum emulsion gel exhibited good rheology and stability, which imparted functionality and health value to the cream. The embedding process of Ganoderma polysaccharide was optimized using one-way and response surface experiments, and the results showed that the embedding rate of Ganoderma polysaccharide reached a maximum value of 93.21% when beeswax was added at 2.3%, 58% of oil phase, the emulsion temperature was 76℃, and the homogenization time was 5.7 min. The properties of Ganoderma polysaccharide beeswax-based water-in-oil emulsion gels were examined in terms of particle size, texture, potential, and freeze-thaw stability. It was observed that the addition of Ganoderma lucidum polysaccharide emulsion gel to the plant cream resulted in a reduction of particle size and a significant increase in the homogeneity and stability of the emulsion (P<0.05). Texture analysis showed that the hardness of the plant cream increased by 22.58%, viscosity increased by 9.23%, and elasticity increased by 18.42%, with the hardness of the plant cream reaching 14.28 gf. The absolute value of the electrical potential increased to 28.26 mz, and the freeze-thaw stability of the emulsion gel was found to be good. Animal experiments confirmed that mice ingesting Ganoderma lucidum polysaccharide gel showed elevated levels of blood urea nitrogen and whole blood lactic acid content, and decreased levels of hepatic glycogen in a dose-dependent manner, with significant anti-fatigue activity (P<0.05). Ganoderma lucidum polysaccharide emulsion gels were ultimately shown to improve the processing characteristics of vegetable creams and provide a data base for future health food development.
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Funded by the National College Students' scientific and technological innovation and entrepreneurship training program