Antibacterial effect of the essential oil of Litsea cubeba oil against Cronobacter sakazakii
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
To investigate the antibacterial effect of Litsea cubeba oil on wild strains of Cronobacter sakazakii isolated from infant formula milk powder, the antibacterial activity of Litsea cubeba oil against Cronobacter sakazakii was evaluated by continuous double dilution method, plate counting method and growth curve. The antibacterial mechanism was investigated by detecting β-galactosidase activity, alkaline phosphatase activity, intracellular DNA content and bacterial structure changes. The results showed that Litsea cubeba oil had strong antibacterial effect against Cronobacter sakazakii. The MIC of Litsea cubeba oil against Cronobacter sakazakii was 6.62 μg/mL. The growth of Cronobacter sakazakii was significantly inhibited after 1 MIC treatment, and the activity of extracellular alkaline phosphatase and intracellular β-galactosidase in Cronobacter sakazakii were significantly increased by 2 times and 13.50 times as compared with the control group(P < 0.05). Compared with the control group, the cell morphology of the bacteria was destroyed, the contents of the cell were leaked, the amount of nucleic acid and protein leaked was 1.03 times that of the control group (P<0.05), the intracellular DNA content was reduced to 0.17 μg/mL and DAPI fluorescence area was decreased, and the brightness of the gel block electrophoresis bands was darkened, and DNA was damaged. In summary, the essential oil of Litsea cubeba oil could be used as a highly effective bacteriostatic agent for Cronobacter sakazakii by destroying cell membrane and DNA, and interfering with the normal physiological activity of bacteria, and leaded to the death of bacteria. This study provided a theoretical basis for developing the essential oil of Litsea cubeba oil as a new antibacterial agent.
