Screening and Identification of A High Yield Antimicrobial Peptide F1-producing Strain and Biofilm Formation Inhibition in Multidrug-resistant Bacteria
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Abstract:
Lactobacillus paracasei subsp. tolerans FX-6, as the original starting strain, was mutated through 60Co γ-ray irradiation. A strain (Strain 26) producing antimicrobial peptide F1 at high yield was identified from the mutated strains. High similarity between FX-6 and strain 26 was revealed through comparative analysis of their 16S rDNA sequences. The crude extract of milk fermented by Strain 26 was further purified to successfully enrich antimicrobial peptide F1. Bacteriostatic experiments showed that the minimum inhibitory concentration (MIC) of the crude extract of milk fermented by Strain 26 on Escherichia coli was 3.16 mg/mL, which was 75% lower than the MIC of the crudee xtract of milk fermented by FX-6, and the yield of antimicrobial peptide F1 by Strain 26 was 3.03-fold that by FX-6. The results preliminarily indicate a possible positive correlation between the concentration of antimicrobial peptide F1 and the antimicrobial activity of the strain. Our preliminary results have shown that the MIC of antimicrobial peptide F1 against colistin-resistant E. coli SHP45 was 320.0 μg/mL, and the results of this study further demonstrated that the biofilm formation in colistin-resistant E. coli SHP45 was inhibited by over 50% when the concentration of antimicrobial peptide F1 reached 2× MIC. These findings indicate that Strain 26 exhibited a stronger ability to produce antimicrobial peptide F1 than FX-6 and that antimicrobial peptide F1 significantly inhibited biofilm formation in colistin-resistant E. coli SHP45. This study offers insights into obtaining strains producing antimicrobial peptide F1 at high yield and is expected to serve as a material basis for the prevention and control of multidrug-resistant bacterial infections.
