Analysis of functional components and structure of crude polysaccharides of Phellinus igniarius from different origins
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Abstract:
To compare and analyze the difference of functional component content and structure of crude Phellinus igniarius polysaccharide from different origins (Jilin, Hebei, Yunnan and Xizang), the crude polysaccharides of Phellinus igniarius were extracted by hot water extraction and ethanol precipitation. The composition of monosaccharides, the preliminary characterization of structural and the content of active components were analyzed by high performance liquid chromatography, Fourier infrared spectrometer and ultraviolet-visible spectrophotometer. The results showed that the crude polysaccharide from Xizang Phellinus igniarius were had the highest content of functional components , the contents of polysaccharides, total phenols, total flavonoids and total triterpenes were 58.81%, 11.11%, 20.96%, 4.49%, respectively. It had the best thermal stability, and the residual amount after thermogravimetric analysis was the highest, and the mass fraction was 51.30%. The crude polysaccharides of Phellinus igniarius were mainly composed of glucose, mannose and galactose. There were O-H, C-H and other characteristic peaks of polysaccharides in the spectra of polysaccharides, and there were acid polysaccharides, and there was no significant difference between in crystal state. This study compared and analyzed the crude polysaccharides of Phellinus igniarius from four sources, and found that the crude polysaccharides of Xizang Phellinus igniarius had the highest content of four effective components and thermal stability. The chemical composition and structure of crude polysaccharides from different places were certain different. This study provided reference for the development and utilization of crude polysaccharides from different origins.
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Project Supported:
The Central Government Guided Local Science and Technology Development Special Project (2023EGA122), Key R&D Plan of Hubei Province (2021BGD016)
