Ultrasonic Coupled Subcritical Water Extration of Lentinus Edodes Polyphenol and Inhibition of α-Glucosidase
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Abstract:
In this paper, the inhibition effect of Lentinus edodes polyphenol on α-glucosidase is investigated by ultrasonic coupled subcritical water method, and the inhibitory effect of Lentinus edodes polyphenol hypoglycemia in vitro was analyzed by fluorescence spectrometry. The results showed that the semi-inhibitory concentration IC50 value of Lentinus edodes polyphenolon α-glucosidase was 0.41mg/mL, and the inhibitory effect of Lentinus edodes polyphenol on α-glucosidase was dose-dependent in the mass concentration range of 0.1-1.5 mg/mL. In addition, when the mass concentration of Lentinus edodes polyphenol reached 1.5mg/mL, the inhibitory activity reached 79.22%, so that the hypoglycemic effect was similar to acarbose. The results of fluorescence spectrum showed that the inhibition type of Lentinus edodes polyphenol was competitive inhibition, and the inhibitory activity decreased with the increase of temperature (298, 303, 310 K). The endogenous fluorescence of α-glucosidase had a static quenching effect, and the number of binding sites to α-glucosidase was 0.35. In summary, Lentinus edodes polyphenol can bind to α-glucosidase to produce in vitro hypoglycemic activity, which has the potential to develop functional Lentinus edodes products with hypoglycemic properties.
