Rapid detection of quantitative method applied in determination of deoxynivalenol for wheat and its products and the influencing factors analysed
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Abstract:
Rapid quantification of vomitoxin in the edible part of wheat is essential to improve the acquisition efficiency. To evaluate the application effect of DONQ-FAST5 rapid quantification method in the detection of vomitoxin in wheat and its products, quality control samples at three levels of 400, 800, and 1845 μg/kg were selected, and the number of parallel determinations was three. The recoveries of the three quality control samples determined by DONQ-FAST5 were over 91.00% compared with those of HPLC and LC-MS/MS, which met the requirements of GB/T 27404-2008 "Laboratory Quality Control Specification Food Physical and Chemical Testing". Only the RSD of the standard value of 400 μg/kg was 7.87%, and the rest of the RSDs were less than 5.00%, with good precision. Under the same conditions, the effects of sample matrix, dilution and temperature on the DONQ-FAST5 rapid quantification method were analysed by comparing the results with those of HPLC and LC-MS/MS. The results showed that when the dilution factor was 5 times and the experimental temperature was 25~30 ℃, compared with the instrumental analysis method, the DONQ-FAST5 rapid quantitative method was satisfactory in the detection of DON content in whole wheat flour, hulled flour and heart flour. Three methods were used to determine the DON content in 371 wheat samples, and the results were consistent. The DONQ-FAST5 rapid quantification method could incubate 4 samples in each batch at the same time, and could accurately quantify the DON content of 4 samples in 15 minutes, which provided a theoretical basis for real-time monitoring of wheat vomiting toxin content in wheat purchasing enterprises.
