The Protective Effect of Haematococcus pluvialis Spore Powder Against UVB-induced Damage
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Abstract:
The study aims to investigate the protective effect of Haematococcus pluvialis spore powder on photodamage induced by ultraviolet B (UVB) radiation. A UVB-irradiated HaCaT cell damage model was established, and H. pluvialis broken spore powder was used for intervention. Cell proliferation was assessed using the CCK8 assay, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the cells were measured using colorimetric methods. UVB irradiation was used to induce damage in zebrafish, and the effect of adding H. pluvialis spore powder on tail fin area was measured, with acridine orange staining used to determine the extent of cell apoptosis. The results showed that intervention with H. pluvialis spore powder (12.5 μg/mL, 25.0 μg/mL, and 50.0 μg/mL) significantly increased the survival rate of HaCaT cells to 84.47%, 89.44%, and 96.30%, respectively. Treatment with 50.0 μg/mL H. pluvialis broken spore powder increased SOD activity in the cells by 35.48% compared to the model group, and reduced MDA content to 23.05% of the model group. Compared with the model group, 0.125 mg/mL and 0.1875 mg/mL H. pluvialis spore powder significantly increased the tail fin area of UVB-damaged zebrafish by 20.07% and 31.04%, respectively, and reduced the apoptosis rate by 72.15% and 74.59%. H. pluvialis spore powder has significant photoprotective effects, improves UVB-induced oxidative damage, and reduces cell apoptosis, providing a basis for further exploration of its functional activity and potential applications.
