Evaluation of Accuracy of Biochip Kit for Detecting Five Types of Veterinary Drug Residues in Animal-derived Foods
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Abstract:
In the study, the accuracy of the detection results of multiple veterinary drug residues in animal-derived foods was evaluated based on the microarray biochip kit method. The microarray biochip kit method was used to determine the residues of quinolones, tetracyclines, florfenicol, sulfonamides, and trimethoprim in five animal-derived food matrices, including chicken, fish, pork, dairy products, and honey. The detection and quantification limits of the biochip kit method were examined, and the accuracy of the results was evaluated. The results showed that the detection limit and quantification limit of the 5 types of veterinary drug residues in the 5 food matrices all met the requirements. Three matrices including fish, honey, and dairy products were selected for spiking detection, and the spiked recovery rates of the 5 types of veterinary drug residues were in the range of 72.58%~119.87%, with RSD values ranging from 0.21% to 22.49%. Compared with the confirmation method, there were no false positive or false negative results, and the accuracy was good. The results indicate that the biochip kit method is rapid, requires low cost, and has accurate and reliable results and broad application prospects in simultaneous screening of multiple veterinary drug residues in animal-derived foods.
