Enhanced Extraction of Ingerol from Ginger Using Pulsed Electric Fields and Process Optimization
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Abstract:
Pulsed electric field (PEF) is a novel non-thermal physical field processing technology used in the sterilization and enzyme inactivation of fluid foods, presenting an substantial amount of research on its applications. In recent years, the superior cell wall-breaking capability of PEF, known as “electroporation,” has attracted increasing attention, and its ability to enhance wall breaking and intracellular functional component extraction has gradually become a popular research topic. The extraction rates of gingerol from ginger were compared by different extraction processes (traditional solvent extraction technique [TET], ultrasonic-assisted extraction technique [UET], soxhlet extraction technique [SET], and pulsed electric field assisted extraction [PEF]). The parameters for the PEF method of extracting gingerol was then optimized by the response surface method. Results indicated that compared to TET, UET, and SET, the PEF method produced the highest extraction yield of gingerol, reaching 13.36 mg/g•DW. Further steps were taken based on single factor and response surface experiment design, and the optimal process parameters for extracting gingerol from ginger by PEF were obtained as follows: electric field intensity: 2.0 kV/cm, pulse frequency: 22, and pulse width: 19 μs, Under this optimal condition, the extracted amount of gingerol was mg/g•DW, an increase of 49.75% compared to the traditional solvent extraction method (9.81 mg/g•DW). This study shows that PEF is the superior extraction methods in enhancing gingerol extraction from ginger and with great application prospects.
