In Vitro Antioxidant Activity of the Flowers of Rosa laxa Retz. from Xinjiang
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The in vitro antioxidant activities of different polar parts of the flowers of Rosa laxa Retz., a characteristic plant resource of Xinjiang, were studied to provide a reference for the subsequent research of this medicinal material. Extracts of different polar parts of the flower using petroleum ether (E1), dichloromethane (E2), ethyl acetate (E3) and n-butanol (E4) were prepared and their in vitro antioxidant activities were evaluated by investigating their scavenging abilities on DPPH, ABTS+, and hydroxyl radicals. E4 with the highest antioxidant activity among them was used as the research object for subsequent studies. An in vitro oxidative damage model of mouse macrophage RAW264.7 induced by D-galactose was established. The control group, model group, Vc group and E4 groups (low-, medium- and high-dose groups) were set up. The cell survival rate was determined by the CCK-8 method, and the activities of superoxide dismutase, glutathione peroxidase, lactate dehydrogenase and catalase in cells, and the content of malondialdehyde in cells, were determined. The antioxidant activities of different polar parts of the Rosa laxa Retz. flower were in the order of E4>E3>E2>E1, among which E3 and E4 exhibited strong free radical scavenging abilities and showed obvious dose-dependent effects. At an E4 mass concentration of 0.5~10 μg/mL, the survival rate of E4 on RAW264.7 cells was ≥ 80%, with no obvious toxicity detected. The treatments at each dose could significantly promote the survival rate of RAW264.7 cells (P<0.05), and in a dose-dependent manner, increase the activities of SOD, GSH-PX, LDH and CAT in cells while reducing the content of MDA. The above results demonstrated that the flowers of Rosa laxa Retz. possessed an in vitro good antioxidant activity.
