The Anti-aging Effects of Exosomes from Chicken Embryos on Mice
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Abstract:
The anti-aging effects of exosomes from chicken eembryos on mice was carried out. Exosomes from chicken embryos were prepared by ultracentrifugation. The ability to promote hematopoiesis was proven by an in vitro hematopoietic colony formation assay. A model of D-galactose (D-gal) induced aging in mice was constructed, and different concentrations of exosomes from chicken embryos were administered by gavage to mice, and their physiology and behavior-related indicators, and hepatic and renal pathology, were detected. The colony formation assay showed that, compared with the blank group, the volume of burst forming unit-erythroid (BFU-E) colonies in groups with low, middle and high doses of exosomes from chicken embryos was larger, and the number of colonies significantly increased by 63.90%, 59.47% and 68.82% respectively (P<0.01). The animal experiments showed that, compared with the aging model group, the thymus index, the proportion of dwell time on the original platform quadrant, and the grip power of limbs in the high-dose exosome group significantly increased by 71.12%, 54.46% (P<0.05) and 21.21% (P<0.01), respectively, and the number of drops of fatigue meter significantly decreased by 64.89% (P<0.05). In the indices related to antioxidation, the total antioxidant capacity (T-AOC) of blood serum and glutathione (GSH) contents significantly increased, by 60.41%, 396.72% (P<0.01), respectively. The alanine aminotransferase (ALT) significantly decreased by 27.88% (P<0.05). Pathological analysis of the low and high dose groups of exosomes from chick embryos both showed effectiveness in alleviating the hepatic and renal damage caused by D-gal. In summary, exosomes from chicken embryos can significantly promote hematopoiesis, improve brain function and muscle strength in aging mice, reduce inflammation, enhance anti-fatigue and antioxidant abilities, improve the structure and function of major organs such as the liver and kidneys, and thus play a significant role in delaying aging. This article provides a theoretical basis for the development of new products aimed at delaying aging.
