Determination of Albendazole and Its Metabolites Residues in Poultry Eggs by High Performance Liquid Chromatography- quadrurpole/electrostatic Field Orbitrap High-resolution Mass Spectrometry
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Abstract:
A method based on high performance liquid chromatography-quadrurpole/electrostatic field orbitrap high-resolution mass spectrometry (HPLC-Q/Qrbitrap MS) was developed for the determination of albendazole and its metabolites in poultry eggs. The egg samples were extracted with acetonitrile. The obtained extracts were dehydrated by anhydrous sodium sulfate, degreased by n-hexane and purified by nitrogen blowing. The residue was dissolved in methanol-water solution (volume ration: 3:7, V/V), followed by well vortexing then filtration through 0.45-μm membrane filters. A Phenomenex C18 column (100 mm×4.6 mm, 2.6 μm) was used to separate the analytes by gradient elution using acetonitrile-0.1% (volume fraction) formic acid aqueous solution as the mobile phase. MS scanning experiments were performed over the range of m/z 100~1 000 in positive mode. The Target-MS/MS scanning mode was used for the second stage. The collision energy was 30 eV and the separation window m/z was 2.0. The mass number of the target compound was accurate with high response value, and no interference in the to-be-analyzed sample was detected by the external standard method with the secondary ion as quantitative ion. The samples were determined based on external standard method with secondary quantitative ion. Under the established chromatographic conditions, albendazole and the three metabolites were well separated. The detection limits and quantification limits were 3 μg/kg and 10 μg/kg, respectively. Good linear relationship was obtained in the range of 20~200 ng/mL, and the correlation coefficients were all higher than 0.99. Spiking experiments were performed for verification, and the average recoveries at three levels were in the range of 75.9%~97.4% with the relative standard deviations (RSD) in the range of 4.3%~8.5%. The proposed method was simple, with a high recovery rate and good stability, thus, can be used for the determination of albendazole and its metabolites in poultry eggs. The method is of great significance for regulating albendazole veterinary drugs in poultry eggs.
