Chemical Constituents and Antioxidant Activity of Viburnum rhytidophyllum Hemsl
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Abstract:
To determine the antioxidant active site and chemical constituents of Viburnum rhytidophyllum Hemsl. fruits, in this study, the ethanolic extract of Viburnum rhytidophyllum Hemsl. was isolated and purified using a systematic solvent extraction method and various chromatographic techniques, including silica gel, gel chromatography, and preparative high-performance liquid chromatography (HPLC), and the spectral data combined with the high-resolution mass spectra were compared with those in the literature. Meanwhile, the antioxidant activities of the extract and its individual compounds were investigated by the DPPH, ABTS, and PTIO assays. It was found that the ethyl acetate fraction exhibited the highest antioxidant activity, followed by the butanol fraction, with the petroleum ether fraction showing the weakest activity. The 10 monomer compounds were isolated from the ethyl acetate fraction and identified as syringaresinol (1), ethyl caffeate (2), α-tocopherol (3), massonianoside E (4), Mellein (5), 4-O-E-coumaroylarbutin (6), 4-O-Z-coumaroylarbutin (7), 6’-O-caffeoylarbutin (8), catechin (9), and epicatechin (10). Compounds 1~8 belong to the phenylpropanoid class and derivatives, while compounds 9 and 10 are flavonoids. Compounds 1~8 and 10 were isolated for the first time from this plant, and compounds 2~8 were isolated for the first time from the Viburnum genus. All the compounds exhibited different degrees of antioxidant activity. Compounds 2, 9, and 10 exhibited significant antioxidant activities in the ABTS, DPPH, and PTIO assays, compared with Vc (Vitamin C) as the positive control. This study provides a scientific basis for the rational development and utilization of Viburnum rhytidophyllum Hemsl. fruits.
