Simultaneous and Rapid Detection of Three Mycotoxins in Food and Medicine Homologous Materials by Fluorescent Nanoparticle-based Immunochromatography
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Abstract:
An ultra-sensitive lateral flow immunochromatography (LFIC) method based on europium nanoparticles (EuNPs) was established for the simultaneous and rapid detection of aflatoxin B1 (AFB1), zearalenone (ZEN), and ochratoxin A(OTA) in medicine and food homologous materials. By optimizing the key parameters such as the mass concentration of the coated artificial antigen, immobilized line position of the coated artificial antigen, mass concentration of the coated goat antimouse IgG, buffer pH, and immunoreaction time, the developed method could achieve the ultrasensitive and simultaneous identification of three target mycotoxins in one sample after a simple sample pretreatment. This method demonstrated a high specificity and sensitivity. The visual limits of detection of the AFB1, ZEN, and OTA standards were 0.5, 4.0, and 2.0 ng/mL, respectively. Their linear ranges (IC10~IC90) were 0.03~4.61, 0.12~8.47, and 0.09~8.26 ng/mL, respectively. In the detection of mycotoxins in ginseng, astragalus, and angelica samples, the mean spiked recovery rates of the three types of analytes ranged from 90.02% to 109.39%, with a relative standard deviation of less than 8.50%. The coincidence rate between the actual sample and HPLC method was 100%. Therefore, the developed EuNP-LFIC method is suitable for the on-site rapid monitoring of mycotoxins such as AFB1, ZEN, and OTA in medicine and food homologous materials.
