Antioxidant Activity of Tremella Polysaccharide and Its Effect on Fibroblast Protein Gene Expression
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Abstract:
Tremella polysaccharide is the main active substance in Tremella, possessing various functions such as antioxidant and anti-aging effects. The widely occurring chemical contaminant in the environment, benzo[a]pyrene (BaP), can be absorbed through the skin and metabolized, thereby posing a risk of carcinogenicity. In this experiment, the antioxidant activity of crude Tremella polysaccharide was measured in vitro, and then cellular assays were conducted to determine the changes in mRNA expression of collagen (Collagen I, Collagen III), Elastin and matrix metalloproteinases-1 (MMP-1) in fibroblasts under the combined effect of BaP and Tremella polysaccharide, along with the protein content of Collagen I. The anti-aging effect of polysaccharides was also determined. It was found that Tremella polysaccharide had good in vitro antioxidant activity with the highest superoxide anion radical scavenging rate of 70.28%, DPPH scavenging rate of 50.10% and hydroxyl radical scavenging rate of 32.69%. Tremella polysaccharide only at 0.1 μg/mL could effectively inhibit the stimulation of related gene expression by BaP at 20 μmol/L (P<0.05). The expression of Collagen I, Collagen III and Elastin genes increased in a polysaccharide concentration-dependent manner. Under BaP exposure, although collagen gene expression increased to 1.26-fold, the heavily expressed MMP-1 caused the degradation of collagen, therefore, under the effect of Tremella polysaccharide, the content of Collagen-1 decreased to 3.06 μg/L (which was lower than the value of the blank control: 3.48 μg/L). The results indicated that Tremella polysaccharide could effectively inhibit the BaP-induced damage to cells and promote the expression of cell genes (on which the concentration had an important influence).
