Analysis of Contamination, Drug Resistance, and Homology of Pseudomonas aeruginosa Isolated from Barreled Water in Ningxia
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Abstract:
The study objectives were to comprehensively evaluate the pollution situation, drug resistance, and phylogenetic relationship of Pseudomonas aeruginosa strains in Ningxia barreled water production enterprises; identify the pollution source; and improve the product quality. Detection of P. aeruginosa was conducted in water samples from each production link of the enterprise. The isolates in the water samples were identified using a VITEK-2 microbial identification system, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and 16S rRNA gene sequencing. The antimicrobial resistance of P. aeruginosa was assessed using a compact VITEK 2 system, a phylogenetic tree was constructed using 16S rRNA gene sequencing data, and homology was analyzed using MALDI-TOF MS. The identification results of the VITEK-2 system, MALDI-TOF MS, and 16S rRNA gene sequencing were 100% consistent with the identification results of the 24 positive strains isolated using conventional methods. Two of the 24 P. aeruginosa strains, 5-B-1 and 7-C-5, were drug-resistant, and the drug resistance rate was 8.3%, indicating that the drug resistance level of waterborne strains was low. The mass spectrometry results showed that the positive strains from the same area were closely related. The phylogeny analysis showed that the isolates of P. aeruginosa were not related to the geographical region, but the strains of P. aeruginosa from the same source were in the same cluster, and the main source of P. aeruginosa was water. This study provides accumulated data for the contamination traceability of P. aeruginosa in barreled water and the theoretical basis for further research on contamination traceability of bacterial species.
