Abstract:Abstract: In order to explore an efficient and environment-friendly extraction method of flavonoids from qamgur, Xinjiang, five different deep eutectic solvents (DES) were prepared and compared, and the best extraction process was determined by ultrasonic-assisted DES extraction.The effects of DES molar ratio, DES water content ,ultrasonic time, ultrasonic temperature and material-liquid ratio on the content of flavonoids in qamgur were made an on-the-spot investigation by single factor test, and response surface methodology was used to further optimize the extraction conditions.On this basis, the extractive of qamgur was purified by AB-8 macroporous adsorption resin, and the antioxidant activity of qamgur flavonoids in vitro was investigated.The results showed that the optimum extraction conditions were as follows: DES composition of choline chloride-glycerol, DES molar ratio of 1:2, DES water content of 20%, ultrasonic time of 41 min, ultrasonic temperature of 40℃ and solid-liquid ratio of 1:40.Under these conditions, the content of qamgur flavonoids reached 9.66mg/g, and it reached 11.32mg/g after purification, which increased by 17.18%.When the mass concentration of purified qamgur flavonoids solution was 100 μg/mL, the radical scavenging rates of ABTS and DPPH reached 48.24% and 35.20%, the IC50 values were 124.41 μg/ml and 272.98 μg/mL, and the absorbance reached 0.45, which indicated that the antioxidant capacity and total reducing capacity were strong.This study provides scientific basis for the application of qamgur flavonoids as functional active ingredients in food industry.
