Abstract:By constructing a model of BPA-induced proliferation of MCF-7, we observed the effects of royal jelly products fermented by Lactobacillus plantarum N1 on the viability, migratory ability, invasive ability, and cell cycle and apoptosis of model BPA-MCF-7. BPA-MCF-7 were used as a model, and blank, control and drug groups were set up to detect changes in cell viability, cell migration, cell invasion ability and apoptosis and cycle before and after royal jelly fermentation; The changes in hypoglycemic activity of royal jelly before and after fermentation were also tested by ex vivo hypoglycemic activity. Compared with the control group, the cell proliferation ability of unfermented royal jelly and royal jelly ferment decreased, and royal jelly ferment significantly inhibited the growth of BPA-MCF-7. Compared with the control group, the cell migration and invasion rates of royal jelly fermentation were 67.06% and 79.00%, respectively, which could inhibit the invasion and migration ability of BPA-MCF-7. Flow cytometric results showed that royal jelly ferment induced apoptosis in BPA-MCF-7, which was mainly blocked in G2/M phase. In vivo hypoglycemic activity assay revealed that royal jelly fermentation exhibited stronger hypoglycemic activity than royal jelly unfermented. It was concluded that royal jelly could induce apoptosis of BPA-MCF-7 after fermentation, with stronger anticancer activity, and the hypoglycemic activity was also improved, which provided a certain theoretical basis for the development and utilization of royal jelly.
