Abstract:The developmental delay, behavioral disorders and cognitive impairments caused by neurodevelopmental disorders bring enormous pain and disaster to infants and their families. Some active substances that can improve locomotion and neurodevelopment in infants and young children urgently need to be developed. 6"-Sialyllactose (6"-SL) is a human milk oligosaccharide (HMOs).It has the effects of improving intestinal health, anti adhesion, antiviral, and promoting brain development. This study investigated the effect of 6"-sialyllactose on promoting neural development and improving locomotion ability in Caenorhabditis elegans (C.elegans).The main results are as follows: within the range of 0.02-1.0 mg/mL, 0.56mg/ml 6"-SL can effectively prolong the paralysis life of nematodes;We have chosen 0.56 mg/ml 6"-SL to the follow researches based on the practical application of 6"-SL. 6"-SL can significantly improve the head swing frequency,pharyngeal pumping rate of CL4176, and improved average speed of N2. 6"-SL can significantly protect the morphology of cholinergic neurons in C.elegans on day 7 and day 11; In addition,6"-SL reduced the acetylcholinesterase activity by 22.60%±8.0%, while increasing the levels of various neurotransmitters in the nematode body: dopamine (DA) level increased by 148.71%, serotonin (5-HT) level increased by 72.17%, 4-aminobutyric acid (GABA) levels increased by 34.72%, while glutamate (Glu) levels increased by 68.47%. In summary, 6"-SL can effectively improve locomotion ability and neural development, providing theoretical support for the application of 6"-SL in infant formula and health products.
