Abstract:China has abundant indigenous chicken breeds, and different breeds of indigenous chicken vary significantly in terms of nutrition, taste, and flavor. In this study, near-infrared hyperspectral imaging technology was used to study the discrimination method for indigenous chicken breeds. Longyou Partridge Chicken (LPC), Jiangshan Wugu Chicken (JWC) and Silkies Chicken (SC) were used as the research objects. Hyperspectral images of three chicken breeds in the band range of 900~1 700 nm were collected and spectral data were extracted. After preprocessing the spectral data, characteristic wavelengths were selected and PLS and SVM discrimination models were established. The results showed that the Detrending-SNV pre-treatment method was the optimal preprocessing method, with ACCc, ACCp and ACCt being 94.17%, 95% and 94.59%, respectively (which increased by 1.76%, 5.26% and 3.52%, respectively, compared with the full spectrum model). The research results showed that it is feasible to use near-infrared hyperspectral imaging technology to discriminate indigenous chicken breeds.