Abstract:In this paper, ammonium molybdate, eggshell and cetyltrimethyl ammonium bromide were used as raw materials to prepare CaMoO4 with improved performance by adjusting the calcination temperature. Additionally, a CuO/CaMoO4 composite antibacterial agent was synthesized using calcium molybdate, copper sulfate, and sodium borohydride as raw materials. The effects of CuO/CaMoO4 antibacterial dosage, culture time, and copper content on antibacterial activity and the antibacterial effect of CuO/CaMoO4 on Escherichia coli (E. coli) were studied under light and dark reaction conditions. The CuO/CaMoO4 composite antibacterial agent synthesized at 650 ℃ exhibited the best antibacterial efficacy. Moreover, when the copper content was 1.20%, the antibacterial rate could reach 100% under light and dark conditions. Bacteriostatic testing of plastic wrapping material containing CuO/CaMoO4 compound antibacterial agent showed that the agent could effectively inhibit E. coli on food surfaces, and the antibacterial rate remained at 100% even after four days of storage. Furthermore, the CuO/CaMoO4 antibacterial compound displayed excellent biocompatibility. Overall, the compound CuO/CaMoO4 antibacterial agent demonstrated rapid and efficient inhibition of E. coli, thereby ensuring food safety and offering novel insights for food storage practices.