Identification of a Protease Producing Brevibacillus borstelensis Strain and Optimization of Enzyme Production Conditions
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Abstract:
In order to improve the enzyme production efficiency of an environmentally isolated strain, and provide a preliminary experimental basis for the subsequent application of the strain and its protease, the protease production ability of strain S8 was preliminarily detected using the hydrolysis circle method, and its identity as Brevibacillu sborstelensis was confirmed by comparing the 16S rRNA sequences. The optimal culture conditions and medium components were determined through single-factor experiments. Finally, the culture conditions and medium were further optimized using response surface methodology with the help of Plackett-Burman design and the steepest ascent method. The optimization results showed that the optimal conditions are as follows: a fermentation time of 38.70 h, an inoculum size of 1.84% (V/V), a fermentation temperature at 35 ℃, a yeast powder addition of 23.70 g/L, a pancreatic digest of casein addition of 11.70 g/L, and a MgSO4 addition of 20.20 g/L. The resulting enzyme production activity of the strain reached 114.79 U/mL, which was 209.70% higher than that under non-optimized conditions. The results of this study provide scientific data for the subsequent fermentation application of this strain.
