Abstract:The chemical composition and antitumor activity of ethyl acetate extracts from Ganoderma lucidum compounds (GCE) were investigated. GCE was extracted using ethyl acetate from compounds of Ganoderma lucidum, Cuscuta chinensis Lam, Schisandra chinensis, and Codonopsis pilosula (at a mass ratio of 50.3:25.2:15.7:8.8). Ultra-high pressure liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used to analyze its composition. Blank, model, positive control, and GCE-treated groups (low, medium, and high dosages) were established. The tumor inhibition rate, organ index, splenic lymphocyte proliferation capacity, and serum cytokine levels were measured. The results showed that GCE is mainly composed of 11 compounds: L-pyroglutamic acid, DL-leucine adenosine, D-erythronolactone, L-norleucine, pyrogallol, 2-hydroxy-3-methylpyran-4-one, chlorogenic acid, 3-(3,4,5-trimethoxyphenyl) propionic acid, quercetin, and schisandrin. The tumor inhibition rates of H22 tumor-bearing mice in low-, medium-, and highdose GCE-treated groups (at 50, 100 and 200 mg/kg) H22 were 35.14%, 38.25%, and 50.87%, respectively. Comparison of the model and treated groups revealed that low-, medium-, and high-dose GCE significantly promoted the proliferation of splenic lymphocytes (P<0.01). A high dose of GCE could significantly promote the secretion of IL-6, TNF-α, and IL-12 in mice (P<0.05). In short, GCE has certain inhibitory effects on the tumor of H22 tumor-bearing mice. The results of this study can provide insights into the development of antitumor products.