Effects of the Free Radicals in Cold Plasma Treatment on the Structure and Allergenicity of Glycinin
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Abstract:
A comparative investigation was performed using oxidation systems comprising single active particles, namely •OH and H2O2, and free radical scavenging systems based on cold plasma (CP) (•OH scavenging: CP + tertiary butanol; H2O2 scavenging: CP+MnO2) to explore the specificity of different active particles in CP treatment (•OH and H2O2) on the structure and antigenicity of glycinin. The IgG/IgE binding abilities of glycinin were found to gradually decrease to 69.00% as the •OH concentration increased up to 20 mmol/L in the •OH oxidation system. In the CP + tertiary butanol oxidation system without •OH, the IgG/IgE binding abilities of glycinin increased from 59.45% (IgG) and 52.50% (IgE) with CP treatment (40 kV, 2 min) alone to 67.95% (IgG) (100 mmol/L tertiary butanol) and 73.77% (IgE) (300 mmol/L tertiary butanol), respectively. Similarly, the IgG /IgE binding abilities of glycinin decreased to 65.65% (IgG) and 30.84% (IgE) as the H2O2 concentration increased to 2.0 mmol/L in the H2O2 oxidation system. In the CP + MnO2 oxidation system without H2O2, the binding abilities of glycinin increased to 91.22% (IgG) and 73.90% (IgE) as the MnO2 concentration increased to 25 mmol/L. Structural analysis based on SDS-PAGE and fluorescence spectra indicate that the •OH-induced primary and tertiary structure changes of glycinin are directly related to the reduction of the antigenicity of glycinin. Meanwhile, only the tertiary structure of glycinin was modified by H2O2. Therefore, •OH and H2O2 are key active particles in CP treatment for the oxidative modification of protein structures and its related antigenicity elimination.
