Enzymatic Preparation and Uric Acid-lowering Effect of Fucoidan Oligosaccharides
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Abstract:
In this study, the optimal conditions for enzymatic hydrolysis of fucoidan oligosaccharides and their uric acid-lowering effect in mice with hyperuricemia were investigated. Fucoidanase (Patent Grant No.ZL201210555581.6) was used to break down fucoidan to obtain fucoidan oligosaccharides, and the optimal enzymatic hydrolysis conditions were explored by taking the inhibition rate of xanthine oxidase as the index. The uric acid-lowering effect was examined by using a hyperuricemia mouse model. The results showed that the optimum enzymatic hydrolysis temperature was 30 ℃ , the optimum enzymatic digestion time was 1.5 h, the optimum enzyme dosage was 2 000 U/g, and the optimum polysaccharide mass concentration was 10 mg/mL. After the enzymatic hydrolysis, the average molecular weight decreased from 7.873×105 u to 8.508×103 u; After the enzymatic hydrolysate was fractionated with an ultrafiltration membrane, the fucoidan oligosaccharides less than 5 ku had the strongest inhibitory effect against xanthine oxidase, with the inhibition rate as 88.65%, sulfate content as 30.93%, fucose content as 33.16%, and glucuronic acid content as 9.29%; Compared with the model group, fucoidan oligosaccharides less than 5 ku could increase the body weights of mice and alleviate the damage to the liver, kidney, and spleen caused by the model drug, and significantly reduce the serum creatinine and urea nitrogen levels of mice. In mice, the treatment with 150 mg/kg and 300 mg/kg fucoidan oligosaccharides decreased the serum uric acid value by 42.10% and 25.63%, respectively, the xanthine oxidase activity by 10.04% and 8.57%, respectively, and the adenosine deaminase activity by 2.83% and 5.25%, respectively. The fucoidan oligosaccharidess less than 5 ku had a significant uric acic-lowering effect in mice with hyperuricemia. This study has laid a theoretical foundation for the development and utilization of fucoidan oligosaccharidess.
