Variation in Dominant Spoilage Bacteria in Daohua Chicken and Establishment of an Arrhenius Shelf-life Prediction Model under Different Storage Temperatures
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Abstract:
To obtain an Arrhenius shelf-life prediction model of Daohua chicken spoilage bacteria, preliminary culture medium screening and complete 16S rDNA gene sequences were used to identify the dominant spoilage bacteria, and the growth changes in the dominant spoilage bacteria and the total number of bacterial colonies under different storage temperatures (25, 4, 0 ℃) were studied. The prediction models for the total number of bacterial colonies and the shelf life of Pseudomonas and Serratia were established and verified based on the kinetic equation of the chemical reaction. The results showed that the dominant spoilage bacteria were Pseudomonas fragi and Serratia liquefaction at different storage temperatures. The shelf life of Daohua chicken under normal-temperature storage at 25 ℃ was less than 0.5 d, and Serratia was dominant in the middle and late stages of decay. The shelf life with refrigeration at 4 ℃ was less than 4 d. Both Pseudomonas and Serratia showed an increasing trend with the extension of storage time. The shelf life with ice storage at 0 ℃ was less than 10 d. There was no significant difference between Pseudomonas and Serratia at the later stage of storage. The shelf-life prediction model was established based on the total number of Pseudomonas and Serratia colonies. The relative errors of the predicted values of the three shelf-life prediction models were all within the permissible range, and the Pseudomonas shelf- life prediction model had the best prediction effect. The results showed that shelf-life prediction models based on the total number of Pseudomonas and Serratia colonies could all predict the shelf life of Daohua chicken.
