Transcriptome Sequencing and Screening of Key Genes Involved in Membrane Lipid Degradation during Dehydration of Thompson Seedless Grape
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Abstract:
In this study, Thompson seedless grapes from Turpan area were used as the experimental materials. After hot-air drying at 25 ℃ and 30 ℃, the grape samples with/without browning that underwent 25%, and 50% of water loss were withdrwan. Transcriptome sequencing technology was used to screen out the key genes related to membrane lipid degradation and metabolism, and real-time fluorescence quantitative PCR technology was used for verification. The results showed that a total of 1.163 billion clean data were obtained by transcriptome sequencing. When the water loss in Thompson seedless grapes was 50%, the comparison between the non-browning and the browning species revealed 718 differentially expressed genes for the rapid dehydration group and 2 259 differentially expressed genes for the slow dehydration group. After the GO function enrichment and KEGG enrichment analyses, 43 differential genes related to membrane lipid metabolism were screened, which were classified into 5 metabolic pathways. Seven key genes related to membrane lipid metabolism were screened from the obtained differential genes, including Aldehyde dehydrogenase 7B4 (ALDH7B4), Digalactose diglycerol synthetase 1 (DGD1), Lipoxygenase (LOX), Lipid phosphate phosphatase 2 (LPP2), Diacylglycerol kinase 5 (DGK5), Non- specific phospholipase C4 (NPC) and Phospholipase Dα1 (PLDα1). qRT-PCR verification confirmed the agreement of the gene expression trend with the transcriptome sequencing results. In conclusion, the changes of membrane lipid degradation and metabolism-related gene expression have influence on dehydration-induced browning in Thompson seedless grapes.
