Regulation Effect of Probiotics on the Intestinal Bacteria in Mice with Antibiotic-associated Diarrhea
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Abstract:
The regulatory effects of probiotics on intestinal flora in antibiotic-associated diarrhea (AAD) mice were investigated. BALB/c mice were randomly divided into 6 groups (8 mice per group). The AAD model was established by intragastric administration of ampicillin 22.4 g/kg, and the blank group was given the same volume of normal saline. After the model was successfully established, the positive group was given bifico (8 g/kg), the low-dose, medium-dose and high- dose probiotics groups (2.5×106, 5×106, 1×107 CFU, respectively) were given compound probiotics, and the model group and blank group were given the same volume of normal saline for 14 consecutive days. The effects of probiotics on the body weight, rate of loose stool, grade of loose stool and diarrhea index in mice, and the serum immunoglobulin IgA and IgG levels, the expression levels of the genes related to intestinal barrier function were determined. The results showed that compared with the AAD model group, the loose stool rate, loose stool grade and diarrhea index of mice of the three probiotics groups all decreased significantly, whilst the the serum IgA and IgG levels increased by 13.30%, 20.25%, 25.83% and 6.84%, 19.81%, 29.64%, respectively, the expression levels decreased by 16.88%, 20.78% and 40.91%, respectively, for TLR4, and by 24.91%, 37.19% and 55.79%, respectively, for NF-κB; the number of viable bacteria of intestinal Bifidobacterium and Lactobacillus increased, whilst the number of viable bacteria of Clostridium perfringens, Enterococcus and Enterobacillus decreased. In conclusion, probiotics can ameliorate AAD by regulating intestinal flora and levels of immunoglobulins.
