Establishment and Application of An Improved QuEChERS Combined with GC-MS/MS Method for the Detection of Six Non-standard Pesticides
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Abstract:
In this study, given the fact that six kinds of pesticides are mainly detected in fruits and vegetables imported and exported from the European Union and Japan, but there are no detection standards in China's national food safety standards, an improved QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction and purification method combined with Gas Chromatography-Tandem Mass Spectrometry (GC-MS/MS) detection method was established. This method could rapidly detect six pesticide residues such as sodium o-phenylphenol and dimethyl dichloromethane in leafy vegetable substrates. The sample was extracted with acetonitrile, salted out with anhydrous MgSO4 and NaCl to remove water, purified with N-propylethylenediamine (PSA) solid-phase material, and analyzed and detected using GC-MS/MS in multiple reaction monitoring (MRM) mode. The results showed that the six pesticides had a good linear relationship in the range from 0.002 to 0.5 μg/mL, with all the correlation coefficient R being greater than 0.998, the detection limit (RSN=3) being 0.15-2.32 μg/kg, the average recovery being 84.50%~106.23%, and the relative standard deviation being 0.96%~8.35%. The results are in accordance with the requirements of the quality control procedures for pesticide residues in the European Union and the pesticide limit requirements of the "uniform standard" for agricultural residues in Japan and the European Union. The established detection method was further applied to the detection of high-incidence pesticide substances in other vegetables, with good detection results. The method established in this study is reproducible, time efficiency, and easy to operate, which provides a new detection technology and basis for the detection of various pesticide residues such as sodium o-phenylphenol and dimethyl dichloromethane.
