Inhibitory Effect of p-Anisaldehyde Against Aspergillus flavus Growth
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Abstract:
The antifungal activity of p-anisaldehyde (AS) against Aspergillus flavus was determined using the two-fold dilution method in 96-well plates. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of AS against A. flavus were found to be 0.80 mg/mL and 3.20 mg/mL, respectively. Spore germination, mycelial biomass synthesis, radial growth, and aflatoxin B1 (AFB1) synthesis were all completely inhibited in A. flavus cultures treated with 2 MIC (1.60 mg/mL) AS. Compared with the control group, the 4 MIC AS group released considerably more intracellular nucleic acid (A260) and protein (A280) within 24 h, as well as exhibited increased extracellular conductivity and pH value. The results of calcofluor white (CW) staining showed that AS treatment had no substantial effect on the cell wall septum of A. flavus. Moreover, 2 MIC AS could effectively inhibit the growth of A. flavus on pistachios, and the total number of molds decreased by 1.89 lg CFU/g on the 8th day. In conclusion, AS treatment substantially inhibited the growth of A. flavus and reduced the mold on pistachios. Therefore, it is expected to be developed as a new fungistatic agent for the food industry.
