Optimization of Inducer Conditions for Promoting Triterpene Production in Liquid State Fermentation of Morchella
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
To fully utilize Morchella resources and increase its triterpene content, the optimal conditions of multiple inducers for promoting triterpene production in liquid fermentation of the fungus were determined using single-factor experiments and response surface methodology. Additionally, the activities of two key enzymes in the triterpene biosynthetic pathway were measured. According to the results obtained, the optimal treatment conditions were a magnetic field intensity of 2.81 mT, gibberellin concentration of 0.56 g/L, and salicylic acid concentration of 214.54 mmol/L. Under these conditions, the maximum concentration of triterpenes accumulated in Morchella was 60.26 mg/g, which was 160.72% (m/m) more than that before optimization. The enzymatic activities of squalene synthase and farnesyl pyrophosphate synthetase were 39.93 and 71.08 IU/L, respectively, which were 190.85% and 178.02% (m/m) (SS) more than that of the control group (FPS). Therefore, combined treatment with a magnetic field, gibberellins, and salicylic acid can considerably promote triterpene production by Morchella. Furthermore, the accumulation of triterpenes was attributed to the enhanced activity of two key enzymes in the triterpene biosynthetic pathway. This study provides a theoretical basis for the development of inducers for enriching beneficial metabolites and new products of Morchella.
