Protective Effect of Arca subcrenata Protein on Probiotics against Antibiotic Stress
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Abstract:
Long-term intake of aquatic products with antibiotic residues can cause intestinal flora homeostasis imbalance. In this study, based on the inhibitory effects of enrofloxacin on four probiotics (Bifidobacterium bifidum BBi32, Lactobacillus rhamnosus LGG, Lactobacillus plantarum LP45 and Bifidobacterium lactis Probio-M8), a probiotic growth model for examining antibiotic stress was established to evaluate the protective effects of crude protein extracts of Nemipterus virgatus, Corbicula fluminea and Arca subcrenata as well as their purification fractions on the enrofloxacin-induced antibiotic stress towards probiotics. Among the four probiotics, Bifidobacterium lactis Probio-M8 showed the highest sensitivity to antibiotic stress. The Arca subcrenatacrude protein extract exhibited a protective effect against antibiotic stress among the three raw materials. Such a crude protein extract was then separated by an anion-exchange column to obtain four fractions (Fr0, Fr1, Fr2 and Fr3) with molecular weights of 14.3~44.3 (Fr0), 20.1~29.0 (Fr1), 14.3~97.2 (Fr2) and <14.3 (Fr3) ku, respectively. Among them, Fr0 showed greater effectiveness in increasing the OD600 of the Probio-M8 culture solution from 0.80 to 1.56, whilst Fr3 significantly shortened the growth generation time of Probio-M8 to 0.98 hour. The Arca subcrenata protein extract promoted the proliferation of Bifidobacterium lactis under enrofloxacin-induced stress; indicating that the protein extract is capable of protecting probiotic bacteria against antibiotic growth stress while promoting probiotic proliferation. This study provides a theoretical basis for the regulatory function of trichothecene protein on intestinal homeostasis and proposes a solution for dealing with antibiotic residues in vivo.
