Polygonatum cyrtonema Hua Extracts Inhibits 3T3-L1 Preadipocytes in Vitro and Alleviates Lipid Accumulation
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The effects of Polygonatum cyrtonema Hua extract on 3T3-L1 preadipocytes and differentiated adipocytes were examined. Raw product extract (RPE), processed product extract (PPE), and polysaccharides (CPP) were obtained using φ=70% ethanol and complex enzymes (cellulase: papain=3:7). Higher saponin, total flavonoid, and total phenol contents (13.63%, 5.37 mg/g, 14.52 mg/g) were obtained in the extract of processed products. Following treatment of 3T3-L1 cells with the extract, it was observed that the preadipocytes were blocked in the G0/G1 phases, thereby inducing apoptosis and inhibiting cell proliferation; in addition, mature adipocytes exhibited significantly less lipid droplet formation and triglyceride (TG) content. PPE at 380 µg/mL had the most remarkable effect on reducing TG content, showing no significant difference compared to commercially available polysaccharide products (PP). Furthermore, the expression of genes associated with lipogenesis and fatty acid oxidation could be regulated, including inhibiting the mRNA expression of PPARγ, C/EBPα, FABP4, and LPL and promoting the mRNA expression of CPT-1. Thus, the findings of this study show that P. cyrtonema extract has the potential to prevent fat accumulation in vitro, suggesting its possible anti-obesity effects.
