Effect of Polygala tenuifolia Alcohol Extracts on Sleep Improvement in Zebrafish
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Abstract:
The effect of different concentrations of Polygala tenuifolia alcohol extracts on sleep improvement in zebrafish was investigated. High-performance liquid chromatography (HPLC) was used to determine the primary active components and their concentrations in P. tenuifolia alcohol extracts. The experimental group was treated with extracts at concentrations of 125, 250, 500, 1 000, and 2 000 μg/mL. After treatment for 1 day at 28 ℃, the number of zebrafish deaths and the mortality rate of zebrafish in each group were observed and counted, and the maximum tolerated concentration (MTC) of samples was determined. According to the MTC, the treatment groups were treated with various concentrations of P. tenuifolia alcohol extracts. In addition to other zebrafish insomnia models, a positive control group (melatonin group) and normal control group were also established. The duration and amount of activity of zebrafish in the awake state were established using a behavior analysis system. MTC was set at 1 000 μg/mL. During the observation period, the sleep improvement effect of the positive melatonin group was 29.00%, and the sleep improvement effects of the four concentration treatment groups (125, 250, 500, 1 000 μg/mL) were 6.97%, 22.78%, 33.14% and 47.26%, respectively. The sleep improvement effect of P. tenuifolia alcohol extract at concentrations of 500 μg/mL and 1 000 μg/mL were significantly superior than that of the positive melatonin group. The findings of this study show that P. tenuifolia alcohol extracts exhibited a dose-dependent improvement effect on PTZ-induced insomnia symptoms in zebrafish, with a more pronounced effect observed at higher concentrations.
