Antioxidant Activity of Quinoa Husks Saponins and Metabolomics-based Investigation of Their Mechanisms of Alleviating Non-alcoholic Fatty Liver Disease
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The in vitro antioxidant activity of total saponins from quinoa husks was explored. Non-alcoholic fatty liver disease (NAFLD) was induced in male SD rats by providing a high-fat diet, and the effects of different doses of crude saponins from husks on hepatic pathology, total cholesterol (TC), triglycerides (TG), serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), and glutathione (GSH) were investigated. The mechanisms of quinoa saponins in preventing NAFLD were revealed by metabolomics studies of urine and liver using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). The results showed that DPPH radical scavenging capacity and ferric ion reducing power of quinoa crude saponins was 86.25% and 0.49, respectively with administration of 0.1~10 mg/mL crude saponin. Animal experiments showed that liver lipids were significantly reduced by supplementation with quinoa crude saponins. TC, TG, ALT, AST, and MDA levels were significantly reduced (P<0.05), and GSH increased (P<0.05). The urine and liver metabolite profiles were altered by quinoa saponins, leading to changes in metabolic pathways, including those associated with glycine and serine metabolism as well as betaine metabolism. In summary, quinoa husk saponins demonstrated notable antioxidant activity in vitro, and significantly reduced hepatic lipid accumulation and antioxidative stress induced by a high-fat diet, suggesting a potential correlation with improvement in multiple metabolic pathways via endogenous metabolite regulation.
