Effect of Black Rice Anthocyanins on Lipid Metabolism Disorders and Intestinal Flora in Food-derived Obese Mice
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Abstract:
The effects of black rice anthocyanin extract (BRE) on the regulation of lipid metabolism and intestinal flora in mice fed a high-fat diet were investigated. In animal experiments, tea polyphenols and low, medium, and high doses of black rice anthocyanin were added to high-fat diets and fed to mice for 8 weeks. Serum levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) levels were measured in the mice, and hepatic gene transcriptome sequencing was used to investigate the mechanisms through which black rice anthocyanin improved lipid metabolism. Finally, 16S sequencing of mouse cecum contents was performed to examine the changes in composition and abundance of intestinal flora. It was found that the serum TG, LDL, and TC levels were reduced by 42.33%, 22.28%, and 11.13%, respectively, whereas HDL levels increased by 30.86% in the high-dose black rice anthocyanin group compared to the high-fat diet group. Black rice anthocyanin intervention also had a significant effect on the mRNA expression levels of genes associated with hepatic lipid metabolism in high-fat diet-fed mice. In addition, the relative abundance of Romboutsia, Muribaculaceae, and Turicibacter was reduced by 56.40%, 64.29%, and 53.53%, respectively. In contrast, the relative abundance of bacteria such as Akkermansia, Rumefaciens, and Lactobacillus increased. This study establishes a crucial foundation for understanding the potential of black rice anthocyanins in ameliorating metabolic diseases such as hyperlipidemia.
