Analysis and Dietary Risk Assessment of Cyromazine Residue in Cowpea
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Abstract:
A simple, rapid and accurate liquid chromatography-tandem mass spectrometry method was developed for the analysis of THE cyromazine residue in cowpea. The extraction conditions including the extraction solvent were optimized. Acetonitrile (containing 15% acetic acid, V%) was used as the extraction solvent, which was purified by the QuEChERS method. The linear range was 0.005~1.00 mg/L, with the limit of detection being 0.001 mg/kg and the limit of quantification being 0.010 mg/kg. The recoveries at 4 spiking levels (0.010, 0.10 0.50 and 1.00 mg/kg) were 76.2%~82.8% with the relative standard deviations in the range of 1.0%~5.7% (n=5), which met the requirements of pesticide residue analysis. The proposed method was used to analyze 112 cowpea samples from the local market, and the detection rate of cyromazine was 22.3%, (4.5% exceeded the standard limit). The results of long-term dietary risk assessment showed that the risk quotient (RQ) was 10.74%, indicating that the cyromazine in cowpea won’t pose an unacceptable risk to the health of the general population. Being stored at room temperature (about 20 ℃) or under cool conditions (4 ℃), the dissipation kinetics of cyromazine residue in cowpea followed the first-order kinetic equation with the half-lives being 5.0 and 7.2 days, respectively. Both the storage temperature and storage time had effects on the change of cyromazine residue in cowpea. Higher temperature would be beneficial to the dissipation of cyromazine residue.
