Analysis of the Non-volatile Differential Compounds of Fresh Leaves and Tea in Taxus by Using UPLC-MS/MS
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Abstract:
In this study, Taxus media fresh leaves (TFL) were processed to Taxus media tea (TT) by fixing, rolling and drying, in order to compare the differential compounds between fresh leaves and tea. A total of 796 compounds were identified both in TFL and TT, with 777 compounds in TFL and 768 compounds in TT. Further, according to the analysis PCA and OPLS-DA, 330 differential compounds were identified, of which 255 compounds such as amino acids, lipids, flavonoids, and phenolic acids were significantly reduced in TT, and 105 compounds such as organic acids were significantly increased in TT. Eleven key differential compounds between TFL and TT were further selected, some of which like citraconic acid, mevalonic acid, (2'S,3'R)-5-(N,N-dimethyl-3'-phenylisosery)-taxachitriene A, 1,3,5-benzenetriol,austrotaxine, 3-hydroxypyridine and pyrrole-2-carboxylic acid were higher in TT. Others like benzaldehyde, hesperetin-7-O-glucoside, 9-Oxo-10E,12Z-octadecadienoic acid, and luteolin-7-O-(6''-caffeoyl) rhamnoside were higher in TFL. This study would provide a theoretical basis for the development and utilization of fresh leaves of Taxus.