Optimization of Compound Enzyme Extraction Process of Asparagus cochinchinensis Polysaccharides: Physicochemical Properties, Hygroscopicity, and Antioxidant Activity
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Abstract:
Response surface methodology was used to optimize the compound enzyme extraction process of Asparagus cochinchinensis crude polysaccharides. The effects of extraction time, temperature, enzyme to substrate mass ratio, and buffer pH on the yield of A. cochinchinensis crude polysaccharides were investigated. Physicochemical indices, antioxidant activity, and hygroscopicity of A. cochinchinensis crude polysaccharides obtained under the optimal conditions were identified. The optimal conditions for compound enzymatic extraction of A. cochinchinensis crude polysaccharides were an extraction time was 4.5 h, extraction temperature of 42.0 ℃, enzyme to substrate mass ratio of 1.5%, and buffer pH of 5.3. The optimized yield of crude polysaccharides was 18.5%, sugar purity of A. cochinchinensis crude polysaccharides reached 95.49%, and protein and polyphenol contents were 1.07% and 0.11%, respectively. Monosaccharide composition analysis revealed that A. cochinchinensis crude polysaccharides mainly consisted of glucose, fructose, fucose, galactose, and xylose in a respective molarratio of 53.91:26.00:6.19:5.33:4.53, and a double helix structure. Hygroscopic analysis revealed a similar moisture absorption rate of A. cochinchinensis crude polysaccharides to that of glycerol, but a markedly higher rate than that of sodium hyaluronate. A. cochinchinensis crude polysaccharides displayed good antioxidant activity. The findings provide a theoretical basis for the enzyme-assisted extraction of highly pure and active A. cochinchinensis crude polysaccharides, which may have valuable potential applications.
