Protective Effect of Moringa oleifera Seed Extract in a Chronic Alcoholic Liver Injury Murine Model Based on NRF2 and NLRP3
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Abstract:
The mechanism underlying the ability of Moringa oleifera seed extract to reduce chronic alcoholic liver injury in mice was anlayzed. The chronic alcoholic liver injury model was established by providing liquid alcohol feed to mice. The intervention group was also administered different concentrations of horseradish seed extract via oral gavage. Subsequently, aspartate aminotransferase (AST), alanine aminotransferase (ALT), interleukin-18 (IL-18), interleukin-1β (IL-1β), malondialdehyde (MDA), reduced glutathione (GSH), and superoxide dismutase (SOD) contents were measured to assess the pathological changes in the mouse liver. Moreover, the expression of specific proteins, namely, nuclear factor crystalloid-related factor 2 (NRF2), HO-1, nucleotide binding oligomeric domain like receptor 3 (NLRP3), and caspase-1 were measured. The results showed that serum AST and ALT levels in the mice with chronic alcoholic liver injury decreased to 71.68 U/L and 46.84 U/L respectively (P<0.05) following the administration of M. oleifera seed extract. Following treatment, the abundance of inflammatory factors (IL-18 wand IL-1β) decreased, whereas the GSH and SOD levels increased in the liver. Moreover, MDA content and NLRP3 and caspase-1 abudance decreased, whereas the expression of NRF2 and HO-1 increased. These findings suggest that M. oleifera seed extract elicits antioxidant and anti-inflammatory effects through the NRF2/HO-1 and NLRP3/caspase-1 signaling pathways.
