Anti-parkinsonism Effect of Laver Combined with Madopar and Its Effect on the Intestinal Flora
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Abstract:
This study aimed to investigate the anti-parkinsonism effect of laver combined with madopar and its influence on the intestinal flora of model mice. Forty-eight C57BL/6J mice were randomly divided into four groups: normal control, Parkinson’s disease (PD) model, madopar treatment, and laver combined with madopar treatment. Mice in the laver combined with madopar treatment group were fed a diet supplemented with 5% laver for 2 weeks. Subsequently, behavioral tests, immunohistochemistry, western blotting, and inflammatory factor level determination were performed to evaluate the brain function of the experimental mice. Paired-end sequencing of intestinal bacterial DNA was conducted on an Illumina platform to monitor changes in the intestinal flora. The results showed that, compared with madopar alone, laver combined with madopar significantly improved the brain function of PD model mice. The number of neurons and expression level of tyrosine hydroxylase in the substantia nigra were 46.95% and 52.91% higher, respectively, while the expression of α-synapse nucleoprotein was 34.56% lower. Serum IL-1β content was significantly lower, and inflammation was lower. Additionally, 16S rRNA sequencing showed that the richness and diversity of the intestinal flora were higher in the laver combined with madopar treatment group than in the madopar alone group. The ratio of Firmicutes/Bacteroidota was 71.59% lower. The relative abundance of Muribaculaceae and Akkermansia, which are related to anti-inflammation, were significantly enriched, whereas the relative abundances of Blautia, Helicobacter, and other inflammatory bacteria were significantly decreased. Compared with madopar alone, combined treatment with laver had more positive effects on the composition of the intestinal flora, reduced inflammation, and exerted stronger anti-parkinsonism effects.
