Research Progress of Molecular Biology Detection Technology for Yersinia enterocolitica
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Abstract:
Yersinia enterocolitica (Y. enterocolitica) is an important food-borne pathogen which can cause human Yersiniosis. The typical symptoms of the disease include diarrhea, ileitis, and mesenteric lymphadenitis. Because of the high detection rate of this microbe in certain foods, such as meat and meat products, it poses a certain threat to food safety. In terms of current detection methods, isolation, purification and biochemical identification methods are time-consuming and difficult to detect Y. enterocolitica at low concentrations in complex food matrices. Therefore, it is urgent to develop a rapid and accurate method to detect Y. enterocolitica in food. In this paper, the existing molecular biology detection methods for Y. enterocolitica are divided into two categories, variable temperature amplification technology and isothermal amplification technology, in order to summarize the most recent relevant research progress. The advantages, disadvantages and development direction are also discussed to provide a reference for the research on the rapid detection method for Y. enterocolitica.
