Magnetic Solid Phase Extraction-gas Chromatography-mass Spectrometry for Analysis of Papaverine in Beverages
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Abstract:
An effective method to detect papaverine in beverages was established based on magnetic solid phase extraction–gas chromatography–mass spectrometry (MSPE-GC-MS). A nanocomposite of magnetic graphene oxide with excellent adsorption properties was successfully synthesized, and its properties were analyzed. The adsorption and desorption conditions (adsorbent dosage, adsorption time, solution pH, elution solvent, and desorption time) were optimized using single-factor experiments, Box-Behnken design (BBD) experiments, and response surface methodology (RSM). The optimal experimental conditions were as follows: magnetic graphene oxide dosage, 15 mg; pH of solution, 7; adsorption time, 30 min; elution solvent, 2 mL methanol ammonium hydroxide (99:1); and desorption time, 6 min. Under optimal conditions, a good linear detection of papaverine was obtained in the range of 0.10~2.00 μg/mL (R2>0.999). The content of papaverine in three beverages was determined using the proposed method. The spike recovery rates of papaverine at three different levels (0.10, 1.00, and 2.00 μg/mL) were 98.43%~105.00%, with relative standard deviations of 0.61%~3.50%. Solid-phase extraction using magnetic graphene oxide combined with GC-MS is a suitable approach for the detection of papaverine. This method not only facilitates efficient and rapid extraction of the target substances, but also ensures quantitative accuracy, with a high recovery rate and excellent reproducibility.
