Construction of Molecularly Imprinted Electrochemical Sensor for Quebrachitol
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Abstract:
A molecularly imprinted electrochemical sensor was prepared using quebrachitol as the template molecule and o-phenylenediamine as functional monomer via electropolymerization on the surface of a glassy carbon electrode modified with nanogold particles/carboxylated carbon nanotubes/chitosan to form a molecularly imprinted film. Scanning electron microscopy examination of the morphology characteristics of the sensor at different modification stages revealed that all the modification materials were modified on the electrode surface. The electrochemical properties of the sensor were studied by of cyclic voltammetry and differential pulse voltammetry, and it was found that the combination of nanogold and carboxylated carbon nanotubes composites could significantly improve the sensitivity of the electrode. The preparation and detection conditions of the molecularly imprinted sensor were optimized, and the results showed that the optimal molar ratio of the template molecule to functional monomer was 1:10, the optimal number of electropolymerization cycles was 25 cycles, and the optimal electropolymerization speed was 100 mV/s, the optimal elution time was 12 min, the optimum adsorption time was 8 min, and the optimum pH of the adsorption solution was 7. Under the optimal experimental conditions, the peak current of the molecularly imprinted sensor had a good linear relationship with the concentration of quercetinol, the detection limi was relatively low, with the recovery rate for the spiked sample in the range of 97.44%~106.19%. The method has good selectivity, reproducibility and stability, which expands the research of electrochemical sensors in the fields of food and medicine.
