Antioxidant Activity of Water Extracts of Caulerpa lentillifera and Their Protective Effects against Oxidative Damage of Human L02 Cells
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Abstract:
To evaluate the antioxidant activity of water extracts of Caulerpa lentillifera (CLE), and to investigate their ability to reduce H2O2-induced oxidative damage in human L02 cells, the composition and content of CLE was measured, and the in vitro antioxidant effects of CLE were evaluated using superoxide anion and hydroxyl radical scavenging experiments. An oxidative damage model of L02 cells was established by H2O2 induction, and the cell counting kit-8 method was used to determine the survival rate after CLE treatment. The activities of extracellular lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) and the concentrations of intracellular malondialdehyde (MDA) and reduced glutathione (GSH) were determined using commercial kits. Fluorescent probes were used to detect changes in the intracellular reactive oxygen species (ROS) levels. The concentrations of total polysaccharides, total triterpenes, total polyphenols, and total flavonoids in radical scavenging abilities, with half-maximal inhibitory concentrations of 0.94 mg/mL and 2.20 mg/mL, respectively. Compared with the model group, the CLE-treated groups (at 22.5 μg/mL and 45.0 μg/mL) demonstrated higher L02 cell survival rate, reduced LDH, AST, and ALT activities in the cell supernatant, lower intracellular ROS levels and MDA concentration, and increased GSH concentration. With the addition of 45.0 μg/mL of CLE, the cell survival rate was significantly increased by 20.09%, whereas the ROS levels and MDA concentrations decreased to 63.23% and 63.20%, respectively, of their concentrations in the model group. Meanwhile, the GSH concentration increased by 164.02%. These results suggest that CLE have strong antioxidant activity in vitro, and possess the ability to mitigate oxidative damage of L02 cells and provide cytoprotective effects.
