Preparation and Characterization of Lotus Root Polysaccharide-chlorogenic Acid Complexes
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Abstract:
To enhance the functionality of lotus root polysaccharides (LRPs) and stabilize chlorogenic acid (CHA) while also facilitating its use, the noncovalent LRP and CHA interactions were studied alongside the physicochemical properties, stability, and bioactivity of the LRP-CHA complex. Based on single-factor and response surface design experiments, the optimal parameters for the preparation of LRP-CHA complexes (measured by CHA binding to LRP) were a temperature of 0 ℃, pH of 5.5, and polyphenol-to-polysaccharide mass ratio of 4:1. These parameters yielded a CHA-to-LRP binding rate of 2 174.01 mg/g and an increase in the average molecular weight from 146.3 ku to 162.1 ku. The IR and UV data indicate noncovalent binding between LRP and CHA. As the dissociation rate of the LRP-CHA complex was found to be positively correlated with temperature, low temperatures are assumed to maintain the stability of the complex. Nonetheless, the dissociation rate was generally less than 25% following simulated oral cavity, stomach, or intestine digestion. The LRP-CHA complex shows dose-dependent scavenging activity for DPPH free radicals, which is indicative of antioxidant activity. Furthermore, the LRP-CHA complex significantly induces NO production in RAW264.7 cells, while also suppressing LPS-stimulated NO production, indicating that it possesses both immunoactivatory and immunosuppressive effects. The findings of this work are expected to serve as theoretical support for the processing and utilization of LRP and CHA.
