Structural Characteristics and Antioxidant Activities of Polygonatum cyrtonema Hua Polysaccharides with Different Origins
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Abstract:
The “water extraction and alcohol precipitation” method was utilized to prepare Polygonatum cyrtonema polysaccharides (PCPs) derived from Polygonatum cyrtonema Hua (PCH) plants grown in five different areas. Comprehensive characterization of the structural features of each PCP was conducted using ion chromatography, high-performance gel-permeation chromatography (HP-GPC), Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometry (XRD), and scanning electron microscopy (SEM). Additionally, the antioxidant activity of each PCP was evaluated. The analyses revealed that the PCP contents varied among the five PCHs, ranging from 7.63% to 16.78%, with statistically significant differences observed in PCP content between the PCHs (P<0.05). Notably, the PCH sourced from Tonggu County (Jiangxi Province) exhibited the highest PCP content (16.78%). All PCPs identified in the study were heteropolysaccharides, with fructose dominating their monosaccharide composition. FTIR spectra of all PCPs displayed characteristic carbohydrate absorption peaks, indicative of α- and β-glycosidic bonds. The micromorphology of PCPs exhibited irregular or porous sheet-like structures. The antioxidant activity assays demonstrated that all five PCPs exhibited varying degrees of antioxidant activity. In the concentration range of 2~10 mg/mL, they exhibited dose-dependent scavenging activities for DPPH and hydroxyl radicals, with the PCP derived from Tonggu County demonstrated the highest antioxidant activity. By revealing the distinct structural characteristics and radical scavenging activities of PCPs from different origins, this study provides a scientific foundation for the breeding and utilization of PCH.
