Preparation, Environmental Tolerance, and Storage Stability of Enteric-soluble Lactobacillus plantarum Microcapsules
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Lactobacillus plantarum ZDY2013 is a strain of lactic acid bacteria that can metabolize complex carbohydrates and help maintain gastrointestinal homeostasis. To improve the survival rate of L. plantarum ZDY2013 in the digestive tract and allow intestine-targeted release, microcapsules were prepared using L. plantarum ZDY2013 and xylo-oligosaccharides as core materials and sodium alginate and whey protein as wall materials. The preparation parameters were optimized using response surface methodology, and the optimized microcapsules were assessed in terms of their ability to withstand extreme environments and gastrointestinal release. The storage stability of the enteric-soluble microcapsules was also evaluated, with result indicating that the optimal microcapsule is prepared using sodium alginate, whey protein, and a calcium chloride mass fractions of 2.0%, 4.0%, and 2.0%, respectively, which results in a microencapsulation rate of 96.67% and survival rate of 95.82%. Microencapsulation improved the survival rate of L. plantarum ZDY2013 by 25.58% and 7.11% in pH 1.5 and 0.45% bile salt conditions (under five hours incubation), respectively. Furthermore, the survival rate of microencapsulated L. plantarum ZDY2013 was 67.63% after 10 minutes in a 65 ℃ hot water bath. The obtained rate of release for the microcapsule was only 0.90% in gastric juice; however, it was 81.90% in colonic fluid, which allows targeted delivery to the intestines. Furthermore, after the microcapsules were stored at -20 ℃ for 60 days, the viable counts of L. plantarum remained above 1010 CFU/g. In summary, the developed enteric-soluble L. plantarum microcapsules have excellent environmental tolerance and can facilitate intestine-targeted release, providing a solid foundation for the functional development of L. plantarum ZDY2013 and xylo-oligosaccharides in specific environments.
